Figure 5
From: Down-regulation of TUFM impairs host cell interaction and virulence by Paracoccidioides brasiliensis
Downregulation of PbTUFM decreases TufM protein on cell surface and in cytosol. Flow-cytometry histograms and immunofluorescent microscopy images of PbWT, PbEV, and PbTufM aRNA non-permeabilized yeast cells (A) and permeabilized yeast cells (B) that were incubated with anti-PbTufM polyclonal serum (1:10). Total of 10,000 cells were analyzed per experiment. (C) Quantification estimate of TufM levels presented as mean of fluorescence. ***p < 0.001 when PbTufM aRNA was compared with PbEV and PbWT. D) Expression levels of TufM in P. brasiliensis surface. One representative blot is shown of three independente experiments. Bar chart shows quantification of protein levels compared to GAPDH control in each strain. **p < 0.05.
