Figure 6

PbTufM aRNA has impairment in the interaction with pneumocytes. (A) The yeast-host cells interaction was evaluated measuring the fluorescence of infected pneumocytes in systems where fungal cells were labeled with CFSE. *p < 0.05; p < 0.005 when comparing PbTufM aRNA with PbWT and PbEV at 8 and 24 h, respectively. (B) The coating of PbTufM aRNA with anti-PbTufM antibody has the potential to influence the pathogen-pneumocytes interaction. Interaction profile of PbTufM aRNA strain with pneumocytes after blocking of PbTufM surface protein with the treatment with anti-PbTufM polyclonal antibody. After treatment with the antibody the yeast cells were labelled with CFSE and then used in the A549 infection. The yeast-host cells interaction was evaluated measuring the fluorescence of infected pneumocytes in systems where fungal cells were labeled with CFSE. *p < 0.05; **p < 0.005; p < 0.0001.