Table 3 Primers designed for antisense oligonucleotide and for hph PCR amplification, and for RT-qPCR analyses.
From: Down-regulation of TUFM impairs host cell interaction and virulence by Paracoccidioides brasiliensis
Oligonucleotides | forward | reverse |
|---|---|---|
PbTufM | 5′ CCGCTCGAGCGGTAAGGCTCCTGAGGAGCGG 3′ | 5′ GGCGCGCCATCAGCGTGTCCGGGGCAGT 3′ |
hph | 5′ AACTCACCGCGACGTCTGTCGA 3′ | 5′ CTACACAGCCATCGGTCCAGA 3′ |
PbTUFM | 5′ TATTCGACCGACAAACGACA 3′ | 5′ TGTTCGCAGCACCAGTAATC 3′ |
β-tubulin | 5′ GTGGACCAGGTGATCGATGT 3′ | 5′ ACCCTGGAGGCAGTCACA 3′ |
