Figure 5

Dynamic cancellous histomorphometry of the proximal tibia, 4th lumbar vertebrae, and femoral neck (n = 8/group). (A) Cancellous bone formation rate was lower in DSS compared to both Con groups in all three bone compartments, with irisin treatment in the DSS group showing modest improvements in BFR at the proximal tibia and femoral neck. (B) Mineralizing surface was lower in DSS compared to both Con groups in all three bone sites, with DSS + Ir demonstrating increased MS/BS compared to DSS alone at the proximal tibia and femoral neck. (C) Mineral apposition rate was decreased in DSS compared to Con groups in the proximal tibia and femoral neck, with a modest increase in MAR in the DSS + Ir group at the proximal tibia and not different from Control groups in the femoral neck. There were not differences at L4. Data are presented as mean ± SD. *Indicates statistically different from all other groups within the bone site (p < 0.05). Bars not sharing the same letter are statistically different within the bone site (p < 0.05).