Figure 7

Immunohistochemistry of osteocytes in the cancellous bone of the distal femur (n = 8/group). (A) %TNF-α-positive osteocytes were higher in DSS compared to all other groups. B) %IL-6-positive osteocytes were higher in DSS compared to all other groups. (C) %RANKL-positive osteocytes were highest in DSS compared to all other groups, while Con + Ir was lower than Con alone and DSS + Ir was not different from Con. (D) %OPG-positive osteocytes were higher in DSS compared to all other groups. (E) %Sclerostin-positive osteocytes were higher in DSS compared to all other groups. (F) %Annexin-V-positive osteocytes were higher in DSS compared to all other groups. (G) Representative images of osteocyte immunohistochemistry (image shown represents sclerostin stain). Data are presented as mean ± SD. *Indicates statistically different from all other groups (p < 0.05). Bars not sharing the same letter are statistically different (p < 0.05).