Figure 1
From: Colorimetric Assay Reports on Acyl Carrier Protein Interactions
The discovery of a colorimetric assay that reports on ACP-KS interactions. While investigating ACP-KS interactions using a previously reported vibrational spectroscopic mechanistic cross-linking approach (blue arrows), an unexpected color change was observed upon mixing the ACP substrate with the KS partner. Upon investigation, it was revealed that the cyanylation reaction had not gone to completion, and thus instead of the ACP-thiocyanate being mixed with KS, the ACP-TNB− complex was inadvertently added. This led to the realization that the facile activation of ACP to ACP-TNB− enables the colorimetric reporting of mechanistically relevant ACP-KS interactions (red arrows).
