Figure 5
From: Colorimetric Assay Reports on Acyl Carrier Protein Interactions
Pre-incubation of E. coli KS FabF with the active site inhibitor cerulenin (0–250 μM) prevents AcpP-TNB− from forming a cross-link with FabF but also causes aggregation at higher concentrations, as observed by non-reducing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) (A) and UVvis. (B) Lanes: 1. Protein standards ladder; 2. AcpP-TNB− (25 μM); 3. FabF (25 μM); 4–9. 25 μM AcpP-TNB− mixed with 25 μM FabF preincubated with 0, 1, 10, 30, 100, and 250 μM cerulenin for 2 hours at room temperature. Inset shows the decrease in A412 versus cerulenin concentration (n = 3 ± standard deviation).
