Figure 8

PCA1 binds to TLR4. (A) RAW264.7 cells treated with for PCA1 (0, 20, 40, 80 μM) for 2 h were incubated with LPS for 2 h. The expression of TLR4 were detected using Western blotting. (B) Treated with PCA1 (80 μM) for 2 h, cells were lysed by RIPA and total proteins were collected. Then, proteins were equally divided into 6 parts, which were heated at 44, 48, 52, 56, 60, and 64 °C for 3 min, respectively. The corresponding loading buffer were added into the proteins, which were analysed by western blotting. The grouping of gels/blots cropped from different parts of the same gel (targets vs loading control). The full blots are shown in Supplementary Information. *p < 0.05, **p < 0.01, and ***p < 0.001 versus the LPS-induced/compared group.