Figure 1 | Scientific Reports

Figure 1

From: Clostridium difficile clade 3 (RT023) have a modified cell surface and contain a large transposable island with novel cargo

Figure 1

PPEP-1 is inactive in RT023 resulting in stable anchoring of sortase substrates to the cell wall. PPEP-1 from RT023 is insoluble in E. coli and inactive in C. difficile. (a) Translated protein sequence alignment of PPEP-1 in 630 and CD305 showing high sequence identity (*) until truncation of the CD305 protein after the putative active site (blue box). (b) Structural prediction of PPEP-1 in 630 and CD305. (c) Expression of 6xHisTag PPEP-1 from 630 and CD305 in E. coli by Coomassie staining and immunoblotting (Mouse anti-His 1:2,000, 680IRDye anti-mouse 1:2,000). U, uninduced; W, whole cell lysate; S, soluble; I, insoluble; FL, full length; Tr, truncated. Samples normalised to an OD 20/ml. (d) Localisation of sortase substrate CD2831 in C. difficile strains 630 and CD305 by Coomassie staining and immunoblotting (Mouse anti-CD2831 1:2,000, 680IRDye anti-mouse 1:2,000). Sup, supernatant; WCL, whole cell lysate. Black arrow indicates CD2831. Samples normalised to OD 50/ml. Full length gels are provided in Supplementary Fig. S1.

Back to article page