Figure 5 | Scientific Reports

Figure 5

From: Clostridium difficile clade 3 (RT023) have a modified cell surface and contain a large transposable island with novel cargo

Figure 5

023_CTnT elements are capable of excising. PCR analysis of CD305 genomic DNA confirmed localisation of 023_CTnT and demonstrated some elements are capable of excising from the genome. (a) Schematic illustrating primer binding to demonstrate element localisation, empty site and circularised sequences. Junctions could be amplified with 1 + 2, 3 + 4, 5 + 6 and 7 + 8. Empty sites could be amplified with 1 + 4, 3 + 6, 5 + 8 and 1 + 8. Circularisation could be amplified with 2 + 3, 4 + 5, 6 + 7 and 2 + 7. (b) PCR analysis of four junctions, empty site and circularisation for 023_CTn1, 023_CTn2, 023_CTn3 and the total site (023_CTnT). +, DNA positive; -, DNA negative. (c) Sequence analysis of the PCR products for 023_CTn3 and 023_CTnT. S, site; C, circularisation; L, left junction; R, right junction.

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