Figure 6 | Scientific Reports

Figure 6

From: Clostridium difficile clade 3 (RT023) have a modified cell surface and contain a large transposable island with novel cargo

Figure 6

Genes within 023_CTnT are expressed in clade 3 strains. RNA extracted from exponential and stationary phase cultures of clade 3 strains CD305, CZ0502 and SLH89 show expression of fourteen genes within 023_CTnT. 16S PCRs were undertaken on RT+ and RT- samples to show uniform production of cDNA and an absence of gDNA respectively. L, ladder; 1, 2, 3 – exponential cultures; 4, 5, 6 – stationary phase cultures; 1, 4 – CD305; 2, 5 – CZ0502; 3, 6 – SLH89; G, genomic DNA from CD305; N, water negative control. CD305 gene ID and putative functions as indicated.

Back to article page