Table 1 Sequences of primers used to amplify different genes by real-time PCR.

From: Uremic serum residue decreases SN-38 sensitivity through suppression of organic anion transporter polypeptide 2B1 in LS-180 colon cancer cells

Target gene (Amplicon size)

Oligonucleotide sequence

Reference

OATP1B1 (248 b.p.)

 

*

     Sense Primer

5′-TGA ATG CCC AAG AGA TGA TG-3′

 

     Antisense Primer

5′-TGG TGG ACC ACT TTA TAC AC-3′

 

OATP1B3 (194 b.p.)

 

*

     Sense Primer

5′-TTG CCG GCC TAA CCT TGA C-3′

 

     Antisense Primer

5′-TGG TTC CCA CTG ACT TTC ATC AC-3′

 

OATP2B1(107 b.p.)

 

28

     Sense Primer

5′-TGA TTG GCT ATG GGG CTA TC-3′

 

     Antisense Primer

5′-CAT ATC CTC AGG GCT GGT GT-3′

 

BCRP (162 b.p.)

 

29

     Sense Primer

5′-GGA TGA GCC TAC AAC TGG CTT-3′

 

     Antisense Primer

5′-CTT CCT GAG GCC AAT AAG GTG-3′

 

MDR1 (188 b.p.)

 

*

     Sense Primer

5′-GTG GTG GGA ACT TTG GCT G-3′

 

     Antisense Primer

5′-TAC CTG GTC ATG TCT TCC TCC-3′

 

MRP2 (83 b.p.)

 

30

     Sense Primer

5′-ACT TGT GAC ATC GGT AGC ATG GA-3′

 

     Antisense Primer

5′-AAG AGG CAG TTT GTG AGG GAT GA-3′

 

β2M (85 b.p.)

 

*

     Sense Primer

5′-TGC TCG CGC TAC TCT CTC TTT C-3′

 

     Antisense Primer

5′-TTC TCT GCT GGA TGA CGT GAG TAA-3′

 
  1. *We synthesized these by using Primer Express® software.
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