Table 1 Details of the sex-chromosome specific-markers used.
Marker | Primer sequences 5′-3′ (fw/rev) | 5′dye | Anneal T (°C) | Primers (μM) | Size (bp) | GenBank Acc. No. |
|---|---|---|---|---|---|---|
AmelY | TTCCAGGCAAGGCTAGAACA | 6-Fam | 47 | 1 | 75 | MK645854 |
ACAACTCAGGGAGGTTTTACG | MK645855 | |||||
SRY | AGCAAGCTGCTGGGATACCAGTG | 6-Fam | 53 | 0.5 | 85 | MK645856 |
TATAGTCCGGGTTCTGCGCCTCC | MK645857 | |||||
PlpX | CTAGCACTGGGTTTGGTTTG | 6-Fam | 50 | 0.5 | 147 | MK654898 |
CCATATCTGCCTCCCTAGAC | MK654899 |
