Figure 7

The levels of IFN-γ (A), IL-10 (B) and IL-5 (C) in vaccinated groups with different formulations: The pooled splenocytes were prepared from three mice in each group (n = 3 per group) and re-stimulated with the mixture of L1 + L2 peptides in vitro. The levels of cytokines were measured in the supernatant with ELISA as mean absorbance at 450 nm ± SD for each sample. All analyses were performed in duplicate for each sample.