Table 4 The selected CTL epitopes of HPV L1 protein based on binding affinity.
Protein Name | Position | Epitope Sequence | No. of Alleles | Top alleles | NetMHCpan Average Rank Scores* | ProPred-I Average Scores** | Syfpeithi Average Scores** | Conservancy (=>75%) | Allergenicity |
|---|---|---|---|---|---|---|---|---|---|
L1 | 12–21 (Type 16) | YLPPVPVSKV | 17 | HLA-A02:01 HLA-A03:01 HLA-B07:02 | 0.574 | 97.324 | 17.285 | Type 31: 100% Type 33: 100% Type 35: 100% Type 51: 100% Type 52: 100% Type 58: 100% Type 56: 90% | Non-allergen |
12–21 (Type 18) | YLPPPSVARV | 17 | HLA-A02:01 HLA-A03:01 HLA-A26:01 | 0.616 | 96.521 | 16.818 | Type 45: 100% Type 39: 90% Type 59: 90% Type 68: 90% | Non-allergen | |
114–125 (Type 16) | GVGISGHPLLNK | 13 | HLA-A03:01 HLA-B58:01 | 0.911 | 61.956 | 13.673 | Type 31: 100% Type 33: 100% Type 35: 100% Type 52: 100% Type 51: 83.33% Type 58: 83.33% | Non-allergen | |
104–115 (Type 18) | GVEIGRGQPLGV | 12 | HLA-B08:01 HLA-B39:01 HLA-B40:01 | 0.831 | 64.810 | 13.678 | Type 59: 100% Type 68: 100% Type 39: 91.67% Type 45: 91.67% Type 51: 91.67% Type 58: 91.67% Type 56: 75% | Non-allergen | |
114–125 (Type 18) | GVGLSGHPFYNK | 11 | HLA-A01:01 HLA-A03:01 HLA-B58:01 | 0.840 | 63.982 | 14.129 | Type 45: 91.67% Type 59: 91.67% Type 51: 83.33% Type 68: 83.33% | Non-allergen | |
411–421 (Type 16) | GGTLEDTYRFV | 14 | HLA-A01:01 HLA-A02:01 HLA-A03:01 | 0.789 | 68.232 | 14.750 | Type 31: 81.82% Type 33: 63.64% Type 35: 81.82% | Non-allergen | |
414–425 (Type 18) | PTTSLVDTYRFV | 14 | HLA-A01:01 HLA-A02:01 HLA-A03:01 | 0.850 | 75.541 | 15.868 | Type 45: 100% Type 59: 91.67% Type 33: 75% Type 52: 75% Type 58: 75% | Non-allergen | |
460–470 (Type 16) | DQFPLGRKFLL | 16 | HLA-B08:01 HLA-B07:02 HLA-A24:02 HLA-A26:01 HLA-B39:01 | 0.757 | 79.497 | 16.523 | Type 31: 100% Type 33: 100% Type 35: 100% Type 39: 100% Type 52: 100% Type 58: 100% Type 59: 100% Type 68: 100% Type 51: 90.91% Type 56: 90.91% Type 18: 81.82% Type 45: 81.82% | Non-allergen | |
461–471 (Type 18) | DQYPLGRKFLV | 17 | HLA-B08:01 HLA-B07:02 HLA-A24:02 HLA-A26:01 HLA-B39:01 | 0.723 | 129.350 | 16.531 | Type 45: 100% Type 16: 81.82% Type 31: 81.82% Type 33: 81.82% Type 35: 81.82% Type 39: 81.82% Type 52: 81.82% Type 56: 81.82% Type 58: 81.82% Type 59: 81.82% Type 68: 81.82% | Non-allergen |
