Table 6 The selected helper T Cell epitopes of HPV L1 protein based on binding affinity.

From: In silico/In vivo analysis of high-risk papillomavirus L1 and L2 conserved sequences for development of cross-subtype prophylactic vaccine

Protein Name

Position

Epitope Sequence

No. of Alleles

Top Alleles

NetMHCIIpan Average Rank Scores*

ProPred II Average Scores**

Conservancy (= > 75%)

Allergenicity

L1

8–22 Type 16

EATVYLPPVPVSKVV

13

DQA:10103

DRB1:1001

DQA:10201

DRB1:0101

6.666

2.735

Type 31: 100%

Type 33: 100%

Type 52: 100%

Type 58: 100%

Type 35: 93.3%

Type 56: 80%

Non-allergen

8–22 Type 18

DNTVYLPPPSVARVV

12

DQA:10501

DRB1:0101

DRB1:1602

6.250

3.180

Type 45: 93.3%

Type 59: 86.6%

Type 68: 86.6%

Type 39: 80%

Non-allergen

95–111 Type 16

TQRLVWACVGVEVGRGQ

10

DRB1:0401

DRB1:0403

DQA:10301

6.630

2.790

Type 39: 100%

Type 31: 94.1%

Type 33: 88.2%

Type 35: 88.2%

Type 58: 88.2%

Type 59: 88.2%

Type 68: 88.2%

Type 51: 88.2%

Type 18: 88.2%

Type 45: 88.2%

Type 52: 82.3%

Type 56: 82.3%

Non-allergen

95–111 Type 18

TQRLVWACAGVEIGRGQ

9

DRB1:0402

DRB1:0403

DQA:10301

6.633

2.720

Type 39: 88.2%

Type 31: 88.2%

Type 33: 88.2%

Type 58: 88.2%

Type 59: 88.2%

Type 68: 88.2%

Type 16: 88.2%

Type 45: 88.2%

Type 52: 88.2%

Non-allergen

327–342 Type 16

NQLFVTVVDTTRSTNM

14

DRB1:0401

DRB1:0405

DRB1:0802

DQA:10201

6.940

2.110

Type 31: 100%

Type 35: 100%

Type 52: 100%

Type 56: 100%

Type 58: 100%

Type 18: 93.7%

Type 33: 93.7%

Type 45: 93.7%

Type 68: 88.2%

Type 39: 87.5%

Type 59: 87.5%

Non-allergen

324–342 Type 18

NQLFVTVVDTTRSTNL

14

DRB1:0401

DRB1:0405

DRB1:0701

DRB1:0802

6.690

2.127

Type 45: 100%

Type 16: 93.7%

Type 31: 93.7%

Type 35: 93.7%

Type 52: 93.7%

Type 56: 93.7%

Type 58: 93.7%

Type 59: 93.7%

Type 33: 87.5%

Type 39: 87.5%

Type 51: 87.5%

Non-allergen

416–430 Type 16

DTYRFVTSQAIACQK

34

DRB1:0701

DRB1:0101

DRB1:0802

DRB1:0401

3.371

5.521

Type 31: 93.3%

Type 33: 93.3%

Type 58: 93.3%

Type 52: 86.6%

Type 18: 80%

Type 35: 80%

Type 51: 76.6%

Non-allergen

417–431 Type 18

DTYRFVQSVAITCQK

34

DRB1:0701

DRB1:0101

DRB1:0802

DRB1:0401

3.385

5.364

Type 45: 93.3%

Type 31: 86.6%

Type 33: 86.6%

Type 52: 86.6%

Type 58: 86.6%

Type 59: 86.6%

Type 68: 80%

Type 16: 80%

Type 56: 76.6%

Non-allergen

  1. *lower rates show better binding affinity, **Higher rates show better binding affinity.
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