Figure 2
From: Uterine SOX17: a key player in human endometrial receptivity and embryo implantation
Localisation of Sox17 to the ‘embryo-endometrial’ interface. Immunohistochemistry of sections of the 3D ECC-1 monolayer/L2-TSC spheroid co-culture model (treated with 10−8M estradiol/progesterone) showed staining localised to the nuclei of ECC-1 cells (A,B). Staining appeared to be upregulated at the point of contact between the L2-TSC spheroid and ECC-1 monolayer. Faint staining only was evident in areas of the ECC-1 monolayer distant from the spheroid adhesion site (C). IgG negative control inset, panel (A). Quantification of immunostaining ‘adjacent’ and ‘distant’ to the site of the adhered spheroid revealed a significant increase in immunostaining intensity ‘adjacent’ to adhesion sites. Data presented as mean ± SEM, n = 6, **p < 0.001.
