Figure 7 | Scientific Reports

Figure 7

From: Characterization, expression profiling, and functional analysis of a Populus trichocarpa defensin gene and its potential as an anti-Agrobacterium rooting medium additive

Figure 7

Transcript-level analysis of jasmonic acid (JA)-related, salicylic acid (SA)-related, pathogenesis-related (PR), and lipid-transfer protein (LTP) genes and JA and SA content between transgenic and wild-type (WT) poplars. (A) Transcript-level analysis of JA- and SA-related genes between transgenic and WT poplars. JA-related genes included LOX, AOC, and HPL. SA-related genes included ICS and PAL. (B) Transcript-level analysis of PR genes between transgenic and WT poplars. (C) Transcript-level analysis of LTP genes between transgenic and WT poplars. Data were analyzed by qRT-PCR and normalized to actin mRNA levels using the 2−ΔΔCt method. Vertical bars represent the means ± standard deviation (n = 3). Significant differences are denoted by asterisks (*P < 0.05; **P < 0.01). Comparison of (D) JA and (E) SA content between transgenic and WT poplars using high-performance liquid chromatography–tandem mass spectrometry. (F) Statistical analysis of differences in JA and SA content between transgenic and WT poplars. Significant differences are denoted by asterisks (*P < 0.05; **P < 0.01).

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