Figure 4
LAMP amplification in paper. (A) Schematic of the method used for retrieving the LAMP amplicons from the paper pads. (B) Gel electrophoresis analysis of products of LAMP reactions conducted in paper starting from varying number of starting Mtb gDNA copies (104 up to 10). The numbers on top of the wells indicate the starting copy number of the template. H- 1000 copies of human gDNA were added to the reaction mix along with 1000 copies of Mtb gDNA; N- No template control; M- DNA marker; and D- digested LAMP amplicons. The reaction time was 40 minutes. Larger spaces between sections of lanes in a gel image indicate lanes from different gels and smaller spaces represent lanes from the same gel. Full-length gels are presented in Supplementary Fig. S9 (iii) and (v).
