Table 2 Millimolar (mM) concentrations of metabolites present in the cell-free supernatants of mMRS containing 1% lactose, L-fucose, 2′-FL or 3-FL, following 24 hours incubation with B. breve UCC2003 WT, B. kashiwanohense APCKJ1 WT, B. breve UCC2003 fumA1-fumST1T2, or with no inoculum (controls). 2′-FL, 2′-fucosyllactose; 3-FL, 3-fucosyllactose; aGluc, α-glucose; ND, not detected.

Culture ID	1,2-propanediol	Ethanol	Lactate	Acetate	Methanol	Formate	L-Fucose	2′-FL	3′-FL	aGluc
No inoculum 1% lactose	ND	ND	3.5 mM	3.8 mM	ND	1.0 mM	ND	ND	ND	1.3 mM
No inoculum 1% fucose	ND	ND	3.8 mM	3.7 mM	ND	1.0 mM	38.4 mM	ND	ND	1.3 mM
No inoculum 1% 2′-FL	ND	ND	3.5 mM	3.7 mM	ND	1.0 mM	ND	20.47 mM	ND	1.3 mM
No inoculum 1% 3-FL	ND	ND	3.5 mM	3.8 mM	1.3 mM	1.0 mM	ND	ND	19.8 mM	1.5 mM
No inoculum mMRS only	ND	ND	3.6 mM	4.0 mM	ND	1.0 mM	ND	ND	ND	1.3 mM
B. breve UCC2003 WT 1% lactose	ND	2.0 mM	6.7 mM	87.0 mM	ND	4.0 mM	ND	ND	ND	1.3 mM
B. breve UCC2003 WT 1% fucose	20.7 mM	6.7 mM	3.4 mM	25.4 mM	ND	19.7 mM	22.1 mM	ND	ND	ND
B. breve UCC2003 WT 1% 2′-FL	ND	1.3 mM	3.4 mM	6.7 mM	ND	1.6 mM	0.6 mM	20.4 mM	ND	1.2 mM
B. breve UCC2003 WT 1% 3-FL	ND	trace	3.4 mM	7.5 mM	1.3 mM	1.6 mM	ND	ND	20.5 mM	2.0 mM
B. kashiwanohense APCKJ1 WT 1% lactose	ND	1.7 mM	6.6 mM	91.7 mM	ND	3.0 mM	ND	ND	ND	1.5 mM
B. kashiwanohense APCKJ1 WT 1% fucose	1.7 mM	2.0 mM	3.3 mM	9.7 mM	ND	5.2 mM	36.1 mM	ND	ND	1.2 mM
B. kashiwanohense APCKJ1 WT 1% 2′FL	4.6 mM	3.8 mM	3.4 mM	62.8 mM	ND	9.0 mM	ND	3.3 mM	ND	0.9 mM
B. kashiwanohense APCKJ1 WT 1% 3′FL	4.6 mM	2.7 mM	6.0 mM	58.9 mM	1.3 mM	8.0 mM	ND	ND	3.5 mM	1.3 mM
B. breve UCC2003 fumA1-fumST1T2 1% lactose	ND	7.9 mM	6.0 mM	84.0 mM	ND	2.8 mM	ND	ND	ND	1.3 mM
B. breve UCC2003 fumA1-fumST1T2 1% fucose	9.8 mM	8.6 mM	3.4 mM	14.5 mM	ND	10.9 mM	38.3 mM	ND	ND	1.2 mM
B. breve UCC2003 fumA1-fumST1T2 1% 2′-FL	5.1 mM	9.0 mM	5.4 mM	61.6 mM	ND	12.0 mM	10.8 mM	ND	ND	1.3 mM
B. breve UCC2003 fumA1-fumST1T2 1% 3-FL	7.8 mM	9.9 mM	4.0 mM	68.2 mM	1.3 mM	15.0 mM	7.6 mM	ND	ND	1.3 mM

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