Table 3 B. breve UCC2003-fumA1-fumST1T2 genes that were transcriptionally upregulated during growth in mMRS medium supplemented with 2′-FL or lactose as the sole carbohydrate, as compared to growth in mMRS supplemented with ribose.

From: Metabolism of the predominant human milk oligosaccharide fucosyllactose by an infant gut commensal

Gene ID

Gene name

Function

2′-FL

Lactose

Bbr_0526

lntR

LacI transcriptional regulator

Bbr_0527

lntP1

ABC transporter permease

2.61

3.81

Bbr_0528

lntP2

ABC transporter permease

3.35

2.12

Bbr_0529

lntA

GH42 Beta-galactosidase

2.48

2.21

Bbr_0530

lntS

ABC transporter solute binding protein

Bbr_1551

lacS

Galactoside symporter

18.72

22.27

Bbr_1552

lacZ6

GH2 Beta-galactosidase

12.98

9.05

Bbr_1553

lacI

LacI transcriptional regulator

Bbr_1740

fumF

L-2-keto-3-deoxy-fuconate aldolase

4.16

Bbr_1741

fumD

L-fuconolactone hydrolase

4.69

Bbr_1742

fumP

L-fucose permease

4.49

Bbr_1743

fumC

L-keto-3-deoxy-fuconate-4-dehydrogenase

3.70

Bbr_1744

fumE

L-fuconate dehydratase

7.12

Bbr_1745

fumR

LacI transcriptional regulator

  1. The level of expression is shown as a fold-value of increase in expression on each carbohydrate, as compared to a ribose control, with a cut-off of a minimum 2.0-fold increase in expression. Microarray data were obtained using B. breve UCC2003-fumA1-fumST1T2 grown on 1% 2′-FL or lactose and were compared with array data obtained when B. breve UCC2003-fumA1-fumST1T2 was grown on ribose as a control. The cut-off point was set at 2.0-fold, with a P value of <0.001. With the - sign are indicated fold values below the cut-off. The predicted functions were determined using a combination of BLASTP71, Pfam (http://pfam.xfam.org) and KEGG (http://www.genome.jp/kegg/) searches.