Table 3 Egg yolk color parameters of quails fed diets containing Pichia pastoris X-33 grown in parboiled rice effluent supplemented with biodiesel glycerol or in YPD medium.

	Time	Treatments			SEM	P-value
	Time	Control	Pichia Effluent	Pichia YPD	SEM	Treatment	Time	Treat*Time
Yolk color (DSM^® color fan)	0–28 d	3.80	3.91	4.10	0.037	0.7723	<0.0001	0.1197
	28–56 d	4.16	4.31	4.23
	56–84 d	3.91	3.73	3.71
	Average	3.95	3.98	4.01
Lightness (L*)	0–28 d	56.81	58.82	57.15	0.297	0.3562	0.0001	0.4229
	28–56 d	59.62	59.86	59.44
	56–84 d	56.18	57.89	58.05
	Average	57.54	58.86	58.21
Redness (a*)	0–28 d	-5.47	-5.58	-5.44	0.059	0.0366	<0.0001	0.1539
	28–56 d	-4.92	-4.76	-5.00
	56–84 d	-5.68^b	-5.98^ab	-6.21^a
	Average	-5.36	-5.44	-5.55
Yellowness (b*)	0–28 d	35.51	36.37	35.18	0.308	0.1573	<0.0001	0.9582
	28–56 d	39.68	40.57	38.59
	56–84 d	36.35	37.00	36.26
	Average	37.18	37.98	36.68

Different superscript letters in the same row indicate significant difference between averages (P < 0.05) by Tukey’s test; SEM = Standard error of the mean. Control = basal diet; Pichia Effluent = basal diet + P. pastoris X-33 grown in parboiled rice effluent supplemented with biodiesel glycerol; Pichia YPD = basal diet + P. pastoris X-33 grown in YPD medium.

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