Table 4 External egg quality parameters of quails fed diets containing Pichia pastoris X-33 grown in parboiled rice effluent supplemented with biodiesel glycerol or in YPD medium.

	Time	Treatments			SEM	P-value
	Time	Control	Pichia Effluent	Pichia YPD	SEM	Treatment	Time	Treat*Time
Specific gravity	0–28 d	1074.80	1075.13	1074.80	3.729	0.4097	0.6756	0.5044
	28–56 d	1069.50	1069.13	1070.80
	56–84 d	1046.00	1077.69	1076.50
	Average	1063.43	1073.98	1074.03
Eggshell weight (g)	0–28 d	1.15	1.14	1.13	0.010	0.5096	0.1717	0.9905
	28–56 d	1.16	1.12	1.13
	56–84 d	1.19	1.18	1.16
	Average	1.17	1.15	1.14
Eggshell ratio (%)	0–28 d	7.88	7.85	7.92	0.048	0.3178	0.0567	0.5900
	28–56 d	7.56	7.41	7.86
	56–84 d	7.83	7.74	7.77
	Average	7.75	7.67	7.85
Eggshell thickness (mm)	0–28 d	0.290	0.287	0.291	0.002	0.7663	0.0511	0.9042
	28–56 d	0.298	0.298	0.307
	56–84 d	0.299	0.297	0.296
	Average	0.296	0.294	0.298

Different superscript letters in the same row indicate significant difference between averages (P < 0.05) by Tukey’s test; SEM = Standard error of the mean. Control = basal diet; Pichia Effluent = basal diet + P. pastoris X-33 grown in parboiled rice effluent supplemented with biodiesel glycerol; Pichia YPD = basal diet + P. pastoris X-33 grown in YPD medium.

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