Figure 2
From: Downregulation of GRK5 hampers the migration of breast cancer cells
Proteomics, migration and invasion analysis of MDA-MB-231 TRIPZ-shGRK5. (A) Scheme of proteomics analysis. Cells were seeded in quintuples and stimulated with 5 µg/ml doxycycline or control every 48 h for 90 h. Subsequently a proteomic analysis utilizing LC-MS was performed. (B) Overview of up and down regulated pathways revealed by GSEA. Gene ontology biological process was utilized as dataset. (C) Migration analysis of MDA-MB-231 TRIPZ-shGRK5 90 h upon stimulation with doxycycline or control using a boyden chamber migration assay. Representative pictures are shown. (D) Invasion analysis utilizing matrigel coated boyden chambers 90 h after stimulation 5 µg/ml doxycycline or control. (E) Western blot analysis of HS-578T, DU-145 and PC-3 72 h upon siGRK5 KD. The presented blots were cropped (F) Migration analysis of HS-578T, DU-145 and PC-3 90 h upon transfection with sc.ctr. or siGRK5. Relative migration or invasion is shown as mean + SD normalized to not induced or sc.ctr. transfected samples (N = 3). A two-tailed Student’s t-test was performed. *p < 0.05, **p < 0.01, ***p < 0.001.
