Figure 4
From: Downregulation of GRK5 hampers the migration of breast cancer cells
Correlation of GRK5 and GRPR and their impact on migration. (A) Expression analysis of GRPR in different breast cancer cell lines utilizing qPCR. The values are depicted as mean + SD in the left panel and as median + range in the right panel. Student’s t-test was used for statistical evaluation. (B) Expression analysis of GRK5, GRPR and GRP 90 h upon induction with 5 µg/ml doxycycline utilizing qPCR. Values are depicted as mean + SD. Two-tailed Student’s t-test was utilized for statistical evaluation. (C) Migration analysis of MDA-MB-231 cells 90 h upon transfection with sc.ctr. or siGRPR with and without stimulation with 200 nM bombesin. Representative pictures of the crystal violet staining are shown at the right panel. Relative migration values are depicted as mean + SD and normalized to sc.ctr. transfected, not stimulated samples. (N = 3). (D) Migration analysis of MDA-MB-231 cells 90 h after transfection with sc. ctr. or siGRK5 and siGRPR, with or without bombesin stimulation using a boyden chamber assay. Representative pictures of the crystal violet staining are shown. The relative migration values are normalized to sc.ctr. transfected, not stimulated samples and depicted as mean + SD (N = 3). For statistical evaluation one-way ANOVA with Dunnett’s Multiple Comparison Test was used. *p < 0.05, **p < 0.01, ***p < 0.001.
