Figure 3

RS imaging of human brain clot of a large vessel origin. (A) White–light optical image (100×) from ROI presented in Fig. 2A with H&E staining; (B) Auto–fluorescence image (integration in the 2100–2500 cm⁻¹ range); (C) RS image of lipids distribution (integration in the 2830–2860 cm⁻¹ range); (D) K–means CA image and average RS spectra of the lipid–rich class. The spectral range of 800–1850 cm⁻¹ was multiplied by factor 8 comparing to higher region for clarity. RS spectra were collected with the use of the 532 nm laser excitation.