Figure 6

FTIR and Raman imaging of a RBCs–rich human brain clot of cardioembolic origin. (A) White–light optical image (20×), H&E staining and FTIR image of proteins distribution; (B) UHCA image, (performed in regions of 914–1800 and 2800–3100 cm⁻¹) with its average second derivative FTIR spectra corresponding to heme–rich class and fibrin–rich class; (C) White–light optical image (x100), H&E staining and Raman integration images (532 nm excitation) of the organic matter (integration in the 2800–3050 cm⁻¹ range) and heme (integration for the 757 cm⁻¹); (D) CA image with average Raman spectra for heme–rich class and fibrin–rich class.