Figure 4

Molecular microbiology analysis of water samples from different water sources in the Kathmandu Valley. Numbers on the x-axis indicate the ID of the different sampling sites as described in Table 1. Data points for qPCR results are an average of technical triplicate samples and error bars indicate the standard deviation. Data points for Illumina NGS sequencing are an average of duplicate samples and error bars indicate the standard deviation. Library preparation for MinION NGS was unsuccessful for the sample from location 10 and thus data are not available (n.a), while the DNA extraction yield for the blank was too low for NGS, and for the sample from location 1, the yield was too low for all molecular microbiology methods.