Figure 5 | Scientific Reports

Figure 5

From: Chemical activation of divergent protein tyrosine phosphatase domains with cyanine-based biarsenicals

Figure 5

AsCy5 targets and activates actPTPκ in a complex cellular proteome. (A) Clarified lysates (0.125 mg/mL) from E. coli expressing wt-PTPκ or actPTPκ were incubated with the indicated concentrations of AsCy5 for 45 minutes, then assayed for PTP activity with pNPP (2 mM, quenched assay). (B) Visualization of targeted proteins from AsCy5-treated cell lysates. Clarified lysates (0.125 mg/mL) expressing actPTPκ or wt-PTPκ were incubated with the indicated concentrations of AsCy5 for 30 minutes. The resulting solutions were diluted by a factor of 75, and 15 µL of each dilution were separated by SDS-PAGE. AsCy5-bound proteins were visualized using near infrared illumination. An uncropped image of the gel is presented in Supplementary Fig. S8.

Back to article page