Figure 6
actSHP2 is strongly activated by AsCy3. (A) PTP activity of actSHP2 (50 nM) was measured with pNPP (2 mM, quenched assay) in the absence (DMSO) or presence of the indicated biarsenicals (1 µM) after 60-minute pre-incubations. (B) Dose dependence of actSHP2 activation. PTP activity of actSHP2 (50 nM) was measured with pNPP (2 mM, quenched assay) in the absence (DMSO) or presence of the indicated concentrations of AsCy3 after 60-min pre-incubations. (C) Time dependence of actSHP2 activation. PTP reaction rates of actSHP2 (50 nM) were measured with pNPP (2 mM, continuous assay) in the absence (DMSO) or presence of AsCy3 (500 nM) after indicated pre-incubation times. (D) The activity of wt-SHP2 and actSHP2 (50 nM) was measured with the phosphopeptide DADEpYLIPQQG (200 µM) as a substrate after 15-min of pre-incubation in the absence (DMSO) or presence of AsCy3 (500 nM).
