Figure 7
Design and characterization of actPTPRR. (A) Primary sequence alignment of the WPD loops of actPTP1B, actPTPκ. actSHP2, actHePTP, and actPTPRR. The position corresponding to K556 of PTPRR is highlighted in red. (B) PTP activity of actPTPRR (200 nM) was measured with pNPP (2 mM, quenched assay) in the absence (DMSO) or presence of the indicated biarsenicals (1 µM) after 60-minute pre-incubations. (C) Structural overlay of WPD loops. The WPD loops of PTP1B in the closed conformation (PDB ID: 1SUG, black)48, PTP1B in the open conformation (PDB ID: 2HNP, red)49, HePTP (PDB ID: 2A3K, purple)50, PTPκ (PDB ID: 2C7S, blue)46, SHP2 (PDB ID: 3B7O, green)50, and PTPRR (PDB ID: 2A8B, orange)47 are modeled using the UCSF Chimera software package.
