Table 2 Kinetic constants of wild-type (wt) PTPκ, actPTPκ, and AsCy5-treated (4 µM) actPTPκ assayed with pNPP.

From: Chemical activation of divergent protein tyrosine phosphatase domains with cyanine-based biarsenicals

Enzyme

kcat [s−1]

KM [mM]

kcat/KM [mM−1s−1]

wt PTPκ + DMSO

1.49 ± 0.04

6.0 ± 0.5

0.26 ± 0.02

actPTPκ + DMSO

1.09 ± 0.04

5.3 ± 0.3

0.21 ± 0.01

actPTPκ + AsCy5

4.1 ± 0.2

3.9 ± 0.2

1.05 ± 0.08

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