Table 3 Kinetic constants of wild-type (wt) SHP2, actSHP2, and AsCy3-treated (1 µM) actSHP2 assayed with pNPP.

From: Chemical activation of divergent protein tyrosine phosphatase domains with cyanine-based biarsenicals

Enzyme

kcat [s−1]

KM[mM]

kcat/KM [mM−1s−1]

wt SHP2 + DMSO

6.8 ± 0.5

4.6 ± 0.7

1.5 ± 0.3

actSHP2 + DMSO

6.5 ± 0.3

4.7 ± 0.4

1.4 ± 0.1

actSHP2 + AsCy3

28 ± 1

1.9 ± 0.3

15 ± 2

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