Figure 5
From: Microarray Embedding/Sectioning for Parallel Analysis of 3D Cell Spheroids
Spheroid (HTB-126) processing effects and sectioning (A) Brightfield image of spheroid in PBS after fixation (B) Same spheroid after processing and embedding in a microarray in paraffin section. (C) Schematic of consecutive sections taken from a single spheroid using the coordinate system in Fig. 3G. (D) The cross-section of a single spheroid (stained with H&E) appearing on eleven consecutive 20 µm sections are digitally stitched together in a single image and labeled consecutively.
