Figure 5 | Scientific Reports

Figure 5

From: Proteomic changes in the milk of water buffaloes (Bubalus bubalis) with subclinical mastitis due to intramammary infection by Staphylococcus aureus and by non-aureus staphylococci

Figure 5

Western immunoblotting validation. Top. Distribution of normalised spectral abundance factor (NSAF) values measured in each sample by shotgun proteomics for (A) cathelicidin proteoforms, (B) S100A8, and (C) haptoglobin. Bottom. Western immunoblotting reactivity of the same samples with (D) anti-pan-cathelicidin, (E) anti-S100A8, and (F) anti-haptoglobin antibodies. SAU: milk samples positive for Staphylococcus aureus. NAS: milk samples positive for non-aureus staphylococci. Neg: culture-negative milk. Sample numbers correspond to those listed in Table 1. One microliter of milk was loaded in each lane. Images were cropped to report relevant information. The original experiment images are reported in Supplementary Fig. 1.

Back to article page