Table 3 Significantly differential proteins in Staphylococcus-positive milk with RSC ≥ 1.5 or RSC ≤ −1.5.

From: Proteomic changes in the milk of water buffaloes (Bubalus bubalis) with subclinical mastitis due to intramammary infection by Staphylococcus aureus and by non-aureus staphylococci

Accession

Description

RSC

Pos/Neg

RSC

SAU/Neg

RSC

NAS/Neg

General

function

P48616

Vimentin*

4.78

4.96

4.58

S

A0A0A7NSG7

Cathelicidin 4*

4.75

4.72

4.78

I

Q1JPB0

Leukocyte elastase inhibitor*

4.09

4.17

4

I

X5I0G0

Cathelicidin 1*

4.04

4.1

3.98

I

C8CF42

Probactenecin 7*

3.91

4.02

3.78

I

P46193

Annexin A1

3.86

4.02

3.67

I

P52176

Matrix metalloproteinase-9*

3.8

3.93

3.66

I

P54228

Cathelicidin 6*

3.67

3.72

3.62

I

A0A0A7V3V9

Cathelicidin 2*

3.64

3.74

3.52

I

E1AHZ7

Protein S100A8*

3.52

3.54

3.51

I

Q92176

Coronin-1A*

3.51

3.51

3.51

I

A5D7D1

Alpha-actinin-4

3.48

3.63

3.31

S

P62803

Histone H4*

3.47

3.67

3.24

S

Q2KJD0

Tubulin beta-5 chain

3.45

3.63

3.25

S

Q0VCM4

Glycogen phosphorylase

3.42

3.43

3.42

CM

Q3MHM5

Tubulin beta-4B chain

3.37

3.49

3.23

S

Q3T149

Heat shock protein beta-1*

3.33

3.39

3.26

PD

Q27991

Myosin-10*

3.31

3.28

3.34

S

L0L830

Cathelicidin 7*

3.26

3.37

3.15

I

P40673

High mobility group protein B2*

3.25

3.43

3.04

I

Q5MAR3

Integrin beta*

3.22

3.48

2.9

I

Q0VCW4

L-serine dehydratase/L-threonine deaminase*

3.09

3.16

3.01

AM

P55052

Fatty acid-binding protein, epidermal*

3.08

3.08

3.08

LM

P02253

Histone H1.2*

3.08

3.06

3.11

S

F6MFD5

Peptidoglycan-recognition protein

3.08

3.26

2.86

I

P62808

Histone H2B type 1

3.04

3.04

3.04

S

Q9TU25

Ras-related C3 botulinum toxin substrate 2

2.97

2.99

2.95

I

Q9TU03

Rho GDP-dissociation inhibitor 2*

2.97

3.13

2.79

S

Q2TBL6

Transaldolase*

2.96

3.05

2.86

CM

V9LY96

Cathelicidin 5*

2.95

3.05

2.84

I

Q2HJ86

Tubulin alpha-1D chain

2.95

3.11

2.76

S

Q3SWX7

Annexin A3*

2.86

3.04

2.65

I

Q3B7N2

Alpha-actinin-1

2.85

3.01

2.68

S

Q5E9F5

Transgelin-2*

2.85

2.83

2.86

S

Q3SYV4

Adenylyl cyclase-associated protein 1

2.84

2.78

2.9

S

Q2KJH4

WD repeat-containing protein 1*

2.81

2.63

2.97

S

P68432

Histone H3.1*

2.79

2.91

2.66

S

A7E3Q8

Plastin-3*

2.79

2.86

2.7

S

P81948

Tubulin alpha-4A chain

2.74

2.93

2.51

S

Q3MHR7

Actin-related protein 2/3 complex subunit 2*

2.68

2.61

2.75

S

A7MB62

Actin-related protein 2

2.62

2.6

2.64

S

Q5E9B1

L-lactate dehydrogenase B chain

2.61

2.69

2.52

CM

M1JVB9

Haptoglobin

2.59

2.68

2.49

I

A0A0C5AGQ3

Lysozyme*

2.59

2.64

2.55

I

Q6B855

Transketolase

2.59

2.65

2.54

CM

P02584

Profilin-1

2.58

2.6

2.56

S

Q5E956

Triosephosphate isomerase

2.53

2.56

2.49

S

P10103

High mobility group protein B1

2.52

2.73

2.28

I

A2I7N1

Serpin A3-5

2.52

2.67

2.35

PD

Q3ZBD7

Glucose-6-phosphate isomerase

2.5

2.59

2.4

CM

P0C0S9

Histone H2A type 1

2.5

2.54

2.45

S

Q3T0P6

Phosphoglycerate kinase 1

2.5

2.53

2.47

CM

P19858

L-lactate dehydrogenase A chain

2.48

2.43

2.52

CM

Q3T114

Ribonuclease UK114*

2.47

2.4

2.53

CP

Q32LE5

Isoaspartyl peptidase/L-asparaginase*

2.46

2.34

2.58

CP

P30922

Chitinase-3-like protein 1

2.45

2.32

2.57

I

Q2HJ60

Heterogeneous nuclear ribonucleoproteins A2/B1*

2.39

2.66

2.06

NM

A5A4L2

Histone H2B*,a

2.38

2.59

 

I

P79136

F-actin-capping protein subunit beta

2.32

2.41

2.23

S

P61157

Actin-related protein 3

2.31

2.27

2.35

S

Q5KR47

Tropomyosin alpha-3 chain

2.31

2.37

2.24

S

P09867

Heterogeneous nuclear ribonucleoprotein A1*

2.3

2.45

2.14

S

A4IF97

Myosin regulatory light chain 12B*

2.27

2.39

2.14

S

A3KMV5

Ubiquitin-like modifier-activating enzyme 1*

2.26

2.57

1.87

PD

Q2HJ57

Coactosin-like protein*

2.22

2.24

2.21

I

Q58CQ2

Actin-related protein 2/3 complex subunit 1B*

2.2

2.22

2.18

S

G3N131

Histone H1.1*

2.19

  

S

P49951

Clathrin heavy chain 1*

2.08

2.22

1.92

S

A5D7A0

EF-hand domain-containing protein D2*

2.08

2.2

1.95

S

Q2TA49

Vasodilator-stimulated phosphoprotein

2.06

2.42

1.58

S

Q3ZC84

Cytosolic non-specific dipeptidase

2.05

2.18

1.91

OM

P10096

Glyceraldehyde-3-phosphate dehydrogenase

2.05

2.14

1.97

CM

P60661

Myosin light polypeptide 6

2.02

2.15

1.87

S

Q3T0D0

Heterogeneous nuclear ribonucleoprotein K*

2.01

2.08

1.94

NM

P33433

Histidine-rich glycoproteina

2.01

2.52

 

I

Q4U5R3

Proteasome activator complex subunit 1

2.01

2.07

1.96

PD

Q32PI5

Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform

1.98

2.29

1.59

S

Q56JZ9

Glia maturation factor gamma*

1.97

2.02

1.92

S

Q0VCX1

Complement C1s subcomponent*

1.96

2.24

1.62

I

Q3SZH7

Leukotriene A-4 hydrolase*

1.96

1.91

2.02

I

P31081

60 kDa heat shock protein, mitochondrial*

1.95

2.12

1.76

I

Q32LP0

Fermitin family homolog 3*

1.94

2.15

1.69

I

D2U6Q0

Serum amyloid A protein*

1.93

1.86

2

I

P62326

Thymosin beta-4*

1.91

2.03

1.78

I

P13752

BOLA class I histocompatibility antigen, alpha chain BL3-6*

1.9

2.08

1.7

I

Q2KJI3

Protein FAM49B*

1.89

1.87

1.92

I

Q762I5

Resistin*

1.89

1.92

1.85

LM

Q76LV2

Heat shock protein HSP 90-alpha

1.88

2.06

1.68

PD

Q3SZ54

Eukaryotic initiation factor 4A-I

1.85

1.82

1.88

GE

Q3MHL4

Adenosylhomocysteinase

1.83

1.82

1.84

AM

P80724

Brain acid soluble protein 1*

1.82

1.61

2

GE

P60712

Actin, cytoplasmic 1

1.8

2.01

1.57

S

J9Q6V1

Glutathione peroxidase*

1.8

2.05

1.5

OM

P55859

Purine nucleoside phosphorylase*

1.78

1.51

2

NM

Q9XSJ4

Alpha-enolase

1.77

1.8

1.75

CM

Q27970

Calpain-1 catalytic subunita

1.77

2.13

 

PD

Q865V6

Macrophage-capping protein*

1.76

1.65

1.86

S

Q9GMB8

Serine–tRNA ligase, cytoplasmica

1.74

2.02

 

AM

Q9BGI1

Peroxiredoxin-5, mitochondrial

1.73

1.92

1.52

OM

P51122

Acidic leucine-rich nuclear phosphoprotein 32 family member Aa

 

1.72

 

CT

Q148J6

Actin-related protein 2/3 complex subunit 4*

1.7

1.71

1.69

S

A4FUA8

F-actin-capping protein subunit alpha-1*

1.68

1.63

1.73

S

P06868

Plasminogen

1.68

1.77

1.58

C

P07589

Fibronectin

1.67

  

I

P13753

BOLA class I histocompatibility antigen, alpha chain BL3-7*,a

1.65

1.96

 

I

O46522

Cytochrome b-245 heavy chaina

1.64

2

 

I

Q3SZI4

14-3-3 protein theta

1.62

1.69

1.55

GE

A6H742

Plastin-1*

1.62

1.54

1.69

S

D2U6Z5

Ceruloplasminb

1.61

 

1.75

IM

Q3T035

Actin-related protein 2/3 complex subunit 3*,a

1.57

1.71

 

S

P20000

Aldehyde dehydrogenase, mitochondrial*,a

1.57

1.71

 

CM

P01030

Complement C4b

1.57

 

1.72

I

P0C0S4

Histone H2A.Za

1.56

1.66

 

S

Q27996

Lysozyme C, tracheal isozyme

1.56

1.51

1.61

I

Q5E9B7

Chloride intracellular channel protein 1

1.55

1.52

1.57

C

P68138

Actin, alpha skeletal musclea

1.54

1.72

 

S

X2IZ01

Signal transducer and activator of transcriptiona

1.54

1.59

 

I

Q3ZBT1

Transitional endoplasmic reticulum ATPasea

1.54

1.68

 

CT

Q2HJG5

Vacuolar protein sorting-associated protein 35a

1.54

1.84

 

I

O97680

Thioredoxina

1.53

1.79

 

OM

P19483

ATP synthase subunit alpha, mitochondrialb

  

1.52

CT

P02676

Fibrinogen beta chaina

 

1.79

 

C

P02672

Fibrinogen alpha chaina

 

1.83

 

I

Q5E9R3

EH domain-containing protein 1a

 

1.78

 

CaM

P00829

ATP synthase subunit beta, mitochondriala

 

1.66

 

OM

P81644

Apolipoprotein A-IIa

 

1.64

 

LM

Q3B7M5

LIM and SH3 domain protein 1a

 

1.61

 

S

A6H7G2

Drebrin-like proteina

 

1.58

 

I

Q3ZBH0

T-complex protein 1 subunit betaa

 

1.57

 

PD

Q3MHL7

T-complex protein 1 subunit zetaa

 

1.52

 

PD

Q7SIH1

Alpha-2-macroglobulina

 

1.52

 

I

P27214

Annexin A11a

 

1.52

 

S

Q3SZV7

Hemopexina

 

1.5

 

IM

P11116

Galectin-1b

  

1.61

I

P35466

Protein S100-A4b

  

1.6

I

O02675

Dihydropyrimidinase-related protein 2*,b

  

1.51

S

Q3T054

GTP-binding nuclear protein Ranb

  

1.61

CT

Q3T0D7

GTP-binding protein SAR1a

−1.51

  

CT

Q71SP7

Fatty acid synthasea

−1.52

−1.8

 

LM

P02638

Protein S100-B

−1.53

  

I

P10790

Fatty acid-binding protein, hearta

 

−1.53

 

LM

M9WP41

BTN1A1a

 

−1.57

 

S

Q17QE5

Calcium and integrin-binding protein 1a

−1.56

−1.85

 

I

Q9N0T1

Stanniocalcin-1a

−1.57

−1.9

 

CaM

A0A0C5GDU2

Myostatina

 

−1.59

 

S

Q6RUS0

Butyrophilinb

−1.6

 

−1.75

LM

G1AQP3

Xanthine dehydrogenase/oxidasea

−1.61

−1.81

 

LM

E9NV81

ATP-binding cassette sub-family G (WHITE) member 2a

−1.62

−1.95

 

LM

Q05927

5′-nucleotidaseb

−1.65

 

−1.88

NM

M4QG36

Insulin induced protein 1a

−1.65

−1.88

 

S

O77588

Procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1a

−1.68

−2.19

 

S

A5PK13

Volume-regulated anion channel subunit LRRC8C

−1.68

  

LM

Q2KIY5

Putative phospholipase B-like 2a

−1.69

−2.24

 

LM

P81127

Gamma-soluble NSF attachment protein

−1.71

  

CT

Q58CY6

Prostamide/prostaglandin F synthaseb

−1.73

 

−1.83

LM

Q06805

Tyrosine-protein kinase receptor Tie-1

−1.73

  

A

P37980

Inorganic pyrophosphatase

−1.74

−1.99

−1.53

LM

Q3T0Q2

Transmembrane protein 59

−1.75

−1.87

−1.63

CT

Q3SYV1

Transmembrane protein 263

−1.77

  

CT

Q3SYS6

Calcineurin B homologous protein 1

−1.81

−1.87

−1.75

CaM

Q58DD4

Syndecan-2a

−1.81

−2.13

 

S

D3K0R6

Plasma membrane calcium-transporting ATPase 4

−1.93

−2.04

−1.82

CaM

C1KGU3

Solute carrier family 11 member 2

−1.93

−2.02

−1.85

CT

Q5GJ77

Glycerol-3-phosphate acyltransferase 1, mitochondrial

−2.01

−2.13

−1.91

LM

A0A088F8E5

Acyl-CoA synthetase short-chain family member 2

−2.08

−2.19

−1.97

LM

P08239

Guanine nucleotide-binding protein G(o) subunit alpha

−2.14

−2.25

−2.03

CT

P08169

Cation-independent mannose-6-phosphate receptor

−2.15

−2.47

−1.88

I

Q3ZBE9

Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating

−2.17

−2.65

−1.8

LM

Q2YDI9

Ferritin, mitochondrial

−2.28

−2.4

−2.17

IM

Q58DW6

Ras-related protein Rab-25

−2.28

−2.39

−2.17

CR

Q3MHW6

Monocarboxylate transporter 1

−2.48

−2.55

−2.41

CM

A0A097P9M4

Long-chain fatty acid-CoA ligase 1

−2.6

−3.2

−2.17

LM

P84466

Lanosterol synthase

−2.71

−2.83

−2.61

LM

Q3MHX6

Protein OS-9a

 

−1.57

 

S

Q2TBX4

Heat shock 70 kDa protein 13a

 

−1.59

 

PD

Q0VD19

Sphingomyelin phosphodiesterasea

 

−1.72

 

LM

Q1RMU3

Prolyl 4-hydroxylase subunit alpha-1a

 

−1.73

 

S

Q5EA88

Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmica

 

−1.8

 

CM

Q0IIG7

Ras-related protein Rab-5Aa

 

−2.11

 

CT

A6QR11

Protein kinase C-binding protein NELL2a

 

−2.37

 

CaM

Q5E9B5

Actin, gamma-enteric smooth muscleb

  

−3.76

S

  1. The table reports the differential proteins obtained when considering all staphylococcus-positive milk samples (Pos/Neg), only SAU-positive milk samples (SAU/Neg) or only NAS-positive milk samples (NAS/Neg). The general functional classification is indicated as follows: A, angiogenesis; AM: aminoacid metabolism; CaM, calcium metabolism; CM, carbohydrate metabolism; CP: catabolic process; CT, cellular transport; C, coagulation; GE: gene expression; I, immunity; IM, iron metabolism; LM: lipid metabolism; NM: nucleotide metabolism; OM: oxidative metabolism; PD, protein degradation; S, structure. Gene ontology results are detailed in Supplementary File 1.
  2. *Detected in staphylococcus-positive milk (≥2 peptide spectrum matches, PSM) and not detected in healthy milk. aSignificantly changed only in SAU-positive milk. bSignificantly changed only in NAS-positive milk.