Figure 1 | Scientific Reports

Figure 1

From: CENP-A binding domains and recombination patterns in horse spermatocytes

Figure 1

Localization of MLH1 foci. (a) Triple immunofluorescence with anti-SCP3 antibody (blue), CREST serum (red) and anti-MLH1 antibody (green) on a horse pachytene spread. The XY body is indicated with an arrow. (b) FISH identification of ECA 11 centromere using the 37cen satellite probe (red) on the same pachytene spread. ECA 2 and ECA 11 are the only two chromosomes lacking 37cen signals. ECA 11 can be recognized because it is shorter than ECA 2. (c) Distribution on p and q arms of MLH1 foci on ECA 11 (red), control metacentric chromosomes of similar size (blue), ECA 2 (grey), all other metacentrics (orange) and acrocentrics (cyan). The distance from the centromere was measured in centimetres.

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