Figure 3
From: CENP-A binding domains and recombination patterns in horse spermatocytes
Number, frequency and signal intensity of DSS centromeres. (a) Number of cells containing no DSS bivalents (green) or at least one DSS bivalent (yellow) using the CREST serum (left, number of cells = 25) and the anti-CENP-A antibody (right, number of cells = 25) on horse TE. (b) Box and whisker plot representing the intensity of signals of single-spotted centromeres (orange), centromeres of the XY body (blue), stretched centromeres (red) and single spots in double-spotted centromeres (yellow). Number of cells = 20. (c) ChIP-seq identification of the centromeric domains on ECA 11 in horse TE. Genomic coordinates (Mb) are reported above. Blue peaks represent read coverage from DNA immunoprecipitated with the CREST serum. The dark profile represents read coverage of the non-immunoprecipitated input sample. (d) Number of DSS per cell in TE, LL and KA. For each individual, the number of cells with 0, 1, 2, 3, 4, 5, 6 and 7 DSS centromeres are reported. H statistic with P value, derived from the Kruskal-Wallis test, is reported below.
