Figure 1

Ultra-wide-field color FP and AF images, histology with H&E staining, and SD-OCT images at one month after intravitreal injection of SI without vitrectomy. One month after injection, no significant changes were observed in FP, AF, histology, or OCT images of rabbit eyes injected with 0.1 mg of SI (A–D). Focal hyperautofluorescent areas were observed in eyes injected with 0.2 mg (F), 0.3 mg (J), and 0.4 mg (N) of SI without vitrectomy. Retinal atrophy was seen via H&E staining and SD-OCT following injection with 0.3 mg (K,L) and 0.4 mg (O,P) of SI. Histology with H&E staining in 0.3 mg and 0.4 mg of SI injection showed not only the photoreceptor layer but also all layers of the retina appeared normal in the non-degenerated area, but loss of the photoreceptor layer and disruption of retinal layers were found in the degenerated area (K,O). In the magnified OCT image (L’,P’, dashed-line box in L,P), the borderline between the degenerated area and non-degenerated area was clearly delineated (L’,P’, red arrow) and the degeneration of the outer retina and retinal thinning were detected in the degenerated area (L’,P’, right side of the red arrow). However, in non-degenerated area, whole retinal layers were relatively intact in OCT image (L’,P’, left side of the red arrow). The green line on infrared FP shows the plane where the SD-OCT images were collected (D,H,L,P). Magnified SD-OCT images are shown (D’,H’,L’,P’; dashed-line box in D,H,L,P).