Figure 3
Repression effect on inflammatory gene expression. (a) NBTI or ITu treatment prior to cordycepin treatment suppresses effect of cordycepin. RAW264.7 cells were treated with 10 µM NBTI, 100 nM ITu or DMSO (Ctrl, control) 15 min prior to cordycepin treatment. The cells were stimulated with LPS 1 h after cordycepin treatment (mean ± SD, n = 3). **p < 0.01; ***p < 0.001 compared to DMSO treatment (labelled as Ctrl). (b) 3′-deoxyinosine exerts same repression effect as cordycepin, although to a lesser degree. RAW264.7 cells were treated with 20 µM cordycepin, 20 µM 3′-deoxyinosine (3DI) or DMSO (Ctrl, control) for 1 h prior to LPS stimulation (mean ± SD, n = 3). **p < 0.01; ***p < 0.001 compared to without compound treatment (labelled as LPS).
