Figure 1 | Scientific Reports

Figure 1

From: Intra- and extracellular β-amyloid overexpression via adeno-associated virus-mediated gene transfer impairs memory and synaptic plasticity in the hippocampus

Figure 1

Expression of Aβ by AAV-BRI-Aβ42 and AAV-UBI-Aβ42 in hippocampal neuronal cell cultures. (a) Total Aβ42 levels in the media after AAV constructs incubation. AAV-BRI-Aβ42: [1] 4.7 × 1010 genome particles/ml, [2] 9.3 × 1010 genome particles/ml, and [3] 2.8 × 1011 genome particles/ml. AAV-UBI-Aβ42: [1] 1.5 × 1010 genome particles/ml, [2] 7.7 × 1010 genome particles/ml, [3] 4.6 × 1011 genome particles/ml. AAV-BRI-Aβ42 [1] and [2] promoted a 37-fold and 465-fold increase and AAV-UBI-Aβ42 [3] promoted an 18-fold increase in Aβ42 levels when compared to those elicited by EGFP. In comparison to AAV-BRI-Aβ42 [2], the concentration [3] promoted a 3-fold decrease in the levels of Aβ42. Compared to EGFP, Aβ 10 mM elicited a 669-fold increase (*p < 0.0001). (b) No significant change was observed in Aβ42 oligomers in the cell media after incubation of either AAV constructs. (c) AAV-BRI-Aβ42 promoted a significant increase in the levels of Aβ42 oligomers in the cell lysate compared to EGFP (*p < 0.0001).

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