Figure 6
Katanin p60 regulates adult dentate gyrus neurogenesis. (A,B) BrdU-positive cells in the SGZ are significantly reduced in the dentate gyrus of p60 heterozygous (+/−) animals. (+/+) n = 28 hippocampi, 3 animals; (+/−) n = 28 hippocampi, 3 animals. (+/+) = 99.663% ± 6.927%; (+/−) = 44.530% ± 6.038%. Independent samples T-test (Two-tailed). Data represented as mean ± S.E.M. ***p < 0.0001. (C,D) Sox2/GFAP co-immunostaining reveals expansion of immature neuronal progenitor cells upon katanin p60 downregulation. (+/+) n = 4; (+/−) n = 3. (+/+) = 0.024 ± 0.002; (+/−) = 0.037 ± 0.004. Independent samples T-test (Two-tailed). Data represented as mean ± S.E.M. *p < 0.05. (E) Sox2/GFAP co-immunostaining reveals a similar expansion of immature neuronal progenitor cells upon depletion of the regulatory katanin p80 subunit. (+/+) n = 3; (Taily/Taily) n = 4. (+/+) = 0.029 ± 0.002; (Taily/Taily) = 0.051 ± 0.006. Mann-Whitney U Test (Two-tailed). Data represented as mean ± S.E.M. **p < 0.01.
