Figure 1
Genetic deletion of S6k1 does not alter body weight or behavioural phenotypes of the R6/2 mouse model of HD. Body weight of male (a) and female (b) mice is shown from 4 to 20 weeks of age. The S6k1−/− mice had reduced body weight compared to their WT littermates but deletion of S6k1 did not affect the rate of weight loss observed in the R6/2 mice. (c) Male and (d) female mice locomotor activity was represented by path length and recorded fortnightly from 6 to 20 weeks of age. S6k1 silencing did not improve the decrease in activity observed in the R6/2 mice. (e) Male and (f) female mice rotarod performance is presented as the average latency to fall and recorded fortnightly from 6 to 20 weeks. Genetic deletion of S6k1 did not ameliorate the impairment in rotarod performance seen in R6/2 mice. (g) Male and (h) female mice average grip strength is presented fortnightly from 6 to 20 weeks. S6k1 deletion did not significantly improve the impairment in average grip strength observed in the R6/2 mice. For all studies, WT, males n = 9 and females n = 9, R6/2, males n = 9 and females n = 11, S6k1−/−, males n = 11 and females n = 8, R6/2 × S6k1−/−, males n = 8 and females n = 7. Data were analysed by general linear model (GLM) with repeated measures and are presented as mean ± SEM.
