Figure 3

Deletion of S6k1 does not reverse the brain weight reduction or the mHTT aggregation observed in R6/2 mice. (a) Male and (b) female mouse brain weight was measured at 14 and 20 weeks. As the S6k1^−/− mice have smaller brains compared to WT mice data are represented as % of appropriate control, R6/2 to WT and R6/2 × S6k1^−/− to S6k1^−/−. Deletion of S6k1 did not modify the brain weight loss in R6/2 mice as the % reduction in brain weight was equivalent for R6/2 and R6/2 × S6k1^−/− mice. For 14 weeks, males n = 4–9/genotype and females n = 4–5/genotype and for 20 weeks, males n = 12–14/genotype and females n = 14/genotype. Data were analysed by GLM univariate with Bonferroni correction. ns = non-significant and P > 0.999 for all. (c–f) The Seprion ligand based ELISA assay was used to quantify mHTT aggregation in the striatum, cortex and brain stem of (a, c and e) male and (b, d and f) female R6/2 and R6/2 × S6k1^−/− mice. Aggregate levels increase with age but are not modified by S6k1 deletion. For 14 weeks, males n = 4/genotype and females n = 4–6/genotype and for 20 weeks, males n = 4–7/genotype and females n = 4–8/genotype. Data were analysed with GLM univariate with Bonferroni correction and are presented as mean ± SEM. *P <0.05, **P < 0.01, ***P < 0.005 and ****P < 0.001.