Figure 7
Convulxin, a toxin from Crotalus durissus terrificus venom, does not induce plasma extravasation in the skin. (a) Platelet aggregation was measured turbidometrically using an aggregometer. A 100-μl aliquot of washed platelets was incubated with rhodocytin or convulxin at the indicated concentrations in a cuvette in the aggregometer at 37 °C under constant stirring. (b) Representative images of C48/80 (10 μg/20 μl)-induced, rhodocytin (5 μM/L/20 μl)-induced or convulxin (5 μM/L/20 μl)-induced plasma extravasation in wild-type mice (color), and digitized images used for density value evaluations (black and white) (left panels). Quantitative analysis of the images in the left panel (right panel) (n = 5). (c) ATP release from platelets derived from wild-type (WT) mice in the presence or absence of C48/80, rhodocytin, or convulxin, as determined using an ATP bioluminescence assay. (d) Representative images of rhodocytin (5 μmol/L/20 μl)-induced plasma extravasation with or without the GPIIb/IIIa inhibitor GRGDS (1 μmol/L/20 μl) in wild-type mice (color), and digitized images used for density value evaluations (black and white) (left panels). Quantitative analysis of the images in the left panel (right panel). Values represent means ± SD. One-way ANOVA with Bonferroni’s test: *p < 0.05, **p < 0.01 (n = 5). N.S., nonsignificant difference. Similar results were obtained from at least two independent experiments.
