Figure 4
From: A simple and flexible high-throughput method for the study of cardiomyocyte proliferation
Effect of small molecules on cardiomyocyte proliferation. (A) Results of small molecule drug screen on nuclei count over a period of 72 hours, normalized to control, averaged over three independent screens. A value of 1 indicates an equivalent rate of nuclei increase to control cells, that is, there is no apparent change in death or proliferation compared to control. A value of 0 indicates cell death, and a value of 2 indicates a doubling of nuclei count over time compared to control. (B) Top small molecules that, on average, increased nuclei count. Note that these calculations do not account for variance between screens. (C) Middle small molecules that had no observed effect on nuclei count over time. (D) Small molecules that led to 100% cell death reproducibly in each screen. (E) Example time-lapse of dividing cardiomyocyte over 24 h post-small molecule addition. (F) Example time-lapse of cardiomyocytes over 24 h post-small molecule addition. Note no effect on cardiomyocyte proliferation or death. (G) Example time-lapse of cardiomyocytes undergoing cell death after small molecule addition. (H) All small molecule compounds with a mean nuclear count increase greater than 1, sorted from top to bottom by smallest to largest standard error of the mean.
