Figure 3 | Scientific Reports

Figure 3

From: Angiotensin II type 1 receptor-associated protein deficiency attenuates sirtuin1 expression in an immortalised human renal proximal tubule cell line

Figure 3

mRNA expression levels of ATRAP and SIRT1 in ciRPTEC in response to angiotensin II (Ang II) treatment or serum withdrawal. The ciRPTEC were treated with 10−6 M of Ang II (+) for 24 hours (a,b) or serum withdrawal (−) for 24 hours (c,d). The relative mRNA levels of ATRAP and SIRT1 in the ciRPTEC were determined by RT-qPCR, normalized to 18 S ribosomal RNA. mRNA levels in the absence of Ang II treatment (−; for a,b) or presence of serum (+; for c,d) were set to 1. All data were obtained with three biologically independent experiments. Values represent the means ± standard error. (a) **p < 0.01 vs. Ang II non-stimulation group. (c,d) *p < 0.05, ***p < 0.001 vs. normal serum group. Data were analysed with the unpaired Student’s t-test.

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