Figure 8

Immunofluorescence and flow cytometry studies of GWAT in male offspring at 24 weeks of age. (A) Staining of adipocytes (Caveolin, green) and cluster of macrophage crown-like structures (Mac2, magenta). Groups with postnatal HFD have larger adipocytes and more macrophage crown-like structures. GWAT shows increased crown-like structures with postnatal HFD. The Pre-HFD shows different degrees of crown-like structures (smaller and larger image) (B) GWAT ATM of SVF: Control-ND (n = 18), Pre-ND (n = 12), P/G-ND (n = 4), Control-HFD (n = 24), Pre-HFD (n = 14), P/G-HFD (n = 9) (C) CD11c⁺ ATMs of SVF: Control-ND (n = 18), Pre-ND (n = 12), P/G-ND (n = 4), Control-HFD (n = 24), Pre-HFD (n = 14), P/G-HFD (n = 9) (D) CD11c⁻ ATM of SVF: Control-ND (n = 18), Pre-ND (n = 12), P/G-ND (n = 4), Control-HFD (n = 24), Pre-HFD (n = 14), P/G-HFD (n = 9) *p < 0.05. Flow cytometry of male GWAT at 24 weeks of age show an increase in CD11c⁻ ATMs in P/G group.