Figure 9

Liver morphology and triglyceride content in male offspring. (A) Hematoxylin and eosin (HE) staining of liver of male groups (Control, Pre, P/G) with postnatal ND (top row) and with postnatal HFD (bottom row). There are increased lipid globules with postnatal diet. (B) Comparison of liver triglyceride content adjusted for liver weight (milligram of triglyceride per gram of liver) between the male groups with postnatal HFD. Control-HFD (n = 18), Pre-HFD (n = 15), P/G-HFD (n = 8). (C) Comparison of absolute liver triglyceride content (measured in milligram) between the male groups with postnatal HFD. Control-HFD (n = 18), Pre-HFD (n = 15), P/G-HFD (n = 8). There is no significant difference in liver triglyceride content among groups with postnatal HFD. (D) Quantification of % Area of liver stained with PAS from postnatal HFD groups and (E) representative images PAS staining of liver of postnatal HFD groups.