Table 3 Results of gene specific analysis by quantitative polymerase chain reaction (qPCR) in water samples from Kabetogama Lake Sullivan Bay, Northwest, near Ash River (U.S. Geological Survey Site No. 482542092493701), Voyageurs National Park, MN, USA, 2016.

From: Phytoplankton community and algal toxicity at a recurring bloom in Sullivan Bay, Kabetogama Lake, Minnesota, USA

Date

Genus specific DNA by qPCR (gene copies per 100 milliliters)

Microcystis mcyE

Planktothrix mcyE

Dolichospermum mcyE

Anatoxin-a (anaC)

Saxitoxin (sxtA)

6/21/2016

<390

E2,800

160,000

<960

E560

6/27/2016

E700

<33,000

410,000

2,100

2,800

7/6/2016

E750

47,000

520,000

3,800

1,600

7/12/2016

10,000

<33,000

740,000

5,200

10,000

7/19/2016

12,000

<33,000

640,000

4,500

41,000

7/26/2016

1,200

<33,000

230,000

4,200

14,000

8/1/2016

13,000

62,000

1,200,000

23,000

160,000

8/10/2016

79,000

1,400,000

1,500,000

43,000

1,100,000

8/16/2016

39,000

37,000,000

420,000

95,000

14,000,000

8/23/2016

13,000

3,200,000

530,000

2,800

440,000

8/30/2016

E640

28,000,000

160,000

1,800

42,000

9/13/2016

E400

3,700,000

<1,400

E1,300

E660

  1. [DNA, deoxyribonucleic acid; mcyE, cyanobacterial microcystin synthetase gene copies; anaC, anatoxin-a toxin gene copies; sxtA, saxitoxin toxin gene copies; <, less than or below the detection limit; E, estimated value indicates above the detection limit but below the quantification limit].